{"id":270,"date":"2014-05-26T21:34:04","date_gmt":"2014-05-27T04:34:04","guid":{"rendered":"http:\/\/sop.washington.edu\/?page_id=270"},"modified":"2026-01-21T11:21:40","modified_gmt":"2026-01-21T19:21:40","slug":"mass-spectrometry-center","status":"publish","type":"page","link":"https:\/\/staff.washington.edu\/twentzel\/wordpress\/department-of-medicinal-chemistry\/research\/mass-spectrometry-center\/","title":{"rendered":"Mass Spectrometry Center"},"content":{"rendered":"<div id=\"metaslider-id-14371\" style=\"width: 100%; margin: 0 auto;\" class=\"ml-slider-3-110-0 ml-slider-pro-2-58-0 metaslider metaslider-flex metaslider-14371 ml-slider ms-theme-default nav-hidden\" role=\"region\" aria-label=\"Mass Spec\" data-height=\"350\" data-width=\"600\">\n<div id=\"metaslider_container_14371\">\n<div id=\"metaslider_14371\" class=\"flexslider\">\n<ul class='slides'>\n<li style=\"display: block; width: 100%;\" class=\"slide-33374 ms-image \" aria-roledescription=\"slide\" data-date=\"2025-05-06 07:52:06\" data-filename=\"muK_C_subliMATE_ReX_ScilS-slide.jpg\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"667\" src=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide.jpg\" class=\"slider-14371 slide-33374 msDefaultImage\" alt=\"\" rel=\"\" title=\"muK_C_subliMATE_ReX_ScilS-slide\" srcset=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide.jpg 1000w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide-300x200.jpg 300w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide-768x512.jpg 768w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide-200x133.jpg 200w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide-375x250.jpg 375w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/muK_C_subliMATE_ReX_ScilS-slide-150x100.jpg 150w\" sizes=\"auto, 100vw\" \/><\/li>\n<li style=\"display: none; 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width: 100%;\" class=\"slide-32891 ms-image \" aria-roledescription=\"slide\" data-date=\"2024-10-23 14:12:54\" data-filename=\"My-little-pony.jpg\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" width=\"1500\" height=\"1000\" src=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony.jpg\" class=\"slider-14371 slide-32891 msDefaultImage\" alt=\"image of My-little-pony toy\" rel=\"\" title=\"My-little-pony\" srcset=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony.jpg 1500w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-300x200.jpg 300w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-1024x683.jpg 1024w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-768x512.jpg 768w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-200x133.jpg 200w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-375x250.jpg 375w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-1200x800.jpg 1200w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/My-little-pony-150x100.jpg 150w\" sizes=\"auto, 100vw\" \/><\/li>\n<li style=\"display: none; width: 100%;\" class=\"slide-32892 ms-image \" aria-roledescription=\"slide\" data-date=\"2024-10-23 14:12:54\" data-filename=\"Bruker-TimsTOF-1.jpg\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" width=\"1500\" height=\"1000\" src=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1.jpg\" class=\"slider-14371 slide-32892 msDefaultImage\" alt=\"image of Bruker-TimsTOF lab equipment\" rel=\"\" title=\"Bruker-TimsTOF\" srcset=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1.jpg 1500w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-300x200.jpg 300w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-1024x683.jpg 1024w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-768x512.jpg 768w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-200x133.jpg 200w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-375x250.jpg 375w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-1200x800.jpg 1200w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/Bruker-TimsTOF-1-150x100.jpg 150w\" sizes=\"auto, 100vw\" \/><\/li>\n<li style=\"display: none; width: 100%;\" class=\"slide-32893 ms-image \" aria-roledescription=\"slide\" data-date=\"2024-10-23 14:12:54\" data-filename=\"10192023_K5umCHCA-1.jpg\" data-slide-type=\"image\"><img loading=\"lazy\" decoding=\"async\" width=\"1915\" height=\"943\" src=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1.jpg\" class=\"slider-14371 slide-32893 msDefaultImage\" alt=\"graphic image of kidney structure\" rel=\"\" title=\"10192023_K5umCHCA\" srcset=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1.jpg 1915w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-300x148.jpg 300w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-1024x504.jpg 1024w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-768x378.jpg 768w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-1536x756.jpg 1536w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-200x98.jpg 200w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-375x185.jpg 375w, https:\/\/staff.washington.edu\/twentzel\/wordpress\/wp-content\/uploads\/10192023_K5umCHCA-1-1200x591.jpg 1200w\" sizes=\"auto, 100vw\" \/><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/uwmassspeccenter.bookmylab.com\/users\/login.php?53e4ff6ca1320\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"aligncenter\" src=\"\/wp-content\/uploads\/Reserve-Instruments-button.jpg\" alt=\"Reserve Instruments\" width=\"300\" \/><\/a><\/p>\n<p>Located in I-court (I &#8211; 093) of the Health Sciences Building on the University of Washington campus (<a href=\"\/department-of-medicinal-chemistry\/mass-spectrometry-center\/directions\/\">click here for directions<\/a>), the Mass Spectrometry Center is an open-access self-sustaining instrument laboratory providing a wide variety of mass spectral services to the University and the research community-at-large (including commercial) on a recharge basis.<\/p>\n<h3>The Mass Spectrometry Center offers the following services:<\/h3>\n<ol>\n<li><strong>Instrument Training\u00a0<\/strong>&#8211; Training services available to\u00a0center users:\n<ul>\n<li>Basic UPLC operation and gradient development<\/li>\n<li>Compound optimization for quantitative assays<\/li>\n<li>Basic mass spectrometry analysis on all center platforms<\/li>\n<li>Software training for quantitative and qualitative analysis software<\/li>\n<\/ul>\n<\/li>\n<li><strong>Submitted Sample Analysis\u00a0<\/strong>&#8211; Examples of routine analysis include:\n<ul>\n<li>High resolution accurate mass measurements of small molecules<\/li>\n<li>Lipids, proteins, and small molecules<\/li>\n<li>MALDI-TOF<\/li>\n<li>Protein digest peptide mapping (discovery proteomics)<\/li>\n<li>Whole protein mass determination<\/li>\n<li>LC-MS separations of complex mixtures<\/li>\n<li>GC-MS<\/li>\n<\/ul>\n<\/li>\n<li><strong>Assay\u00a0Development \u00ad<\/strong>&#8211; Contracted development of quantitative (MRM) and qualitative assays:\n<ul>\n<li>Complete quantitative assays including compound optimization and separation methods<\/li>\n<li>Extraction protocol development for quantitative assays including recovery calculations<\/li>\n<li>Calibration curve development for absolute quantification<\/li>\n<\/ul>\n<\/li>\n<li><strong>MRM Quantitative Proteomics\u00a0<\/strong>&#8211;\u00a0We offer support on quantitative proteomics\u00a0including:\n<ul>\n<li>Development of quantification methods and digests for proteins<\/li>\n<li>Quantification in tissues, cells, cell lines, biofluids<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<h3>About the Center<\/h3>\n<p>The Center serves as a resource offering analytical and instructional expertise <a href=\"https:\/\/sop.washington.edu\/department-of-medicinal-chemistry\/research\/mass-spectrometry-center\/contacts\/\">by our excellent staff<\/a> in contemporary mass spectrometric techniques and instrumentation with the primary aim of providing researchers with &#8220;hands-on&#8221; training and ready access to &#8220;state-of-the-art&#8221; instrumentation needed to develop and meet their research interests. Examples of routine analysis are: Low and High Resolution mass analysis, metabolomics, Metid, analyte quantitation, and structural elucidation.<\/p>\n<h3>Recent Publications<\/h3>\n<p><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0306453026000168?via%3Dihub\">Mixed evidence for stress effects on cortisol-testosterone coupling in Syrian refugee and Jordanian non-refugee adolescents<\/a> J. Glass D, Godwin J, Koehn J, Dajani R, Hadfield K, Panter-Brick C, Martin M. <em>Psychoneuroendocrinology. <\/em>2026; Jan 01;186:107757. doi: 10.1016\/j.psyneuen.2026.107757<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40268507\/\">Breastfeeding on Treprostinil: New Data, Lingering Questions.<\/a> Leary PJ, Koehn J, Barros LM, Hogl L, Liston NM, Buber J, Whittington D, Rayner S, Rayner SG. <em>Eur Respir J.<\/em> 2025; Apr 23:2500049. doi: 10.1183\/13993003.00049-2025. Online ahead of print.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39483645\/\">An assessment of a GMP schistosomiasis vaccine (SchistoShield<sup>\u00ae<\/sup>).<\/a> Kim J, Davis J, Lee J, Cho SN, Yang K, Yang J, Bae S, Son J, Kim B, Whittington D, Siddiqui AA, Carter D, Gray SA. <em>Front Trop Dis.<\/em> 2024;5:1404943. doi: 10.3389\/fitd.2024.1404943. Epub 2024 Jul 29.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38849157\/\">DNA-m6A calling and integrated long-read epigenetic and genetic analysis with <em>fibertools<\/em>.<\/a> Jha A, Bohaczuk SC, Mao Y, Ranchalis J, Mallory BJ, Min AT, Hamm MO, Swanson E, Dubocanin D, Finkbeiner C, Li T, Whittington D, Noble WS, Stergachis AB, Vollger MR. <em>Genome Res.<\/em> 2024 Nov 20;34(11):1976-1986. doi: 10.1101\/gr.279095.124.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38581262\/\">P2X<sub>7<\/sub> receptor-dependent increase in endocannabinoid 2-arachidonoyl glycerol production by neuronal cells in culture: Dynamics and mechanism.<\/a> Singh S, Sarroza D, English A, Whittington D, Dong A, Malamas M, Makriyannis A, van der Stelt M, Li Y, Zweifel L, Bruchas MR, Land BB, Stella N. <em>Br J Pharmacol.<\/em> 2024 Aug;181(15):2459-2477. doi: 10.1111\/bph.16348. Epub 2024 Apr 6.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38297307\/\">A pilot study investigating plasma pharmacokinetics and tolerance of oral capecitabine in carcinoma-bearing dogs.<\/a> Wetzel S, Fidel J, Whittington D, Villarino NF. <em>BMC Vet Res.<\/em> 2024 Jan 31;20(1):36. doi: 10.1186\/s12917-023-03805-y.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37730661\/\">Perampanel\u2019s forgiveness factor in a variable medication adherence paradigm in a rat model of chronic epilepsy.<\/a> Guignet M, Campbell A, Vuong J, Whittington D, White HS. <em>J Transl Med.<\/em> 2023 Sep 20;21(1):642. doi: 10.1186\/s12967-023-04490-z.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37131601\/\">DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertools.<\/a> Jha A, Bohaczuk SC, Mao Y, Ranchalis J, Mallory BJ, Min AT, Hamm MO, Swanson E, Dubocanin D, Finkbeiner C, Li T, Whittington D, Noble WS, Stergachis AB, Vollger MR. <em>bioRxiv<\/em> [Preprint]. 2023 Dec 11:2023.04.20.537673. doi: 10.1101\/2023.04.20.537673. Update in: <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38849157\/\">Genome Res. 2024 Nov 20;34(11):1976-1986. doi: 10.1101\/gr.279095.124.<\/a><\/p>\n<p><a class=\"docsum-title\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37131601\/\" data-ga-category=\"result_click\" data-ga-action=\"1\" data-ga-label=\"37131601\" data-full-article-url=\"from_term=Dale+Whittington&amp;from_sort=date&amp;from_pos=1\" data-article-id=\"37131601\">Fibertools: fast and accurate DNA-m6A calling using single-molecule long-read sequencing.<\/a> <span class=\"docsum-authors full-authors\">Jha A, Bohaczuk SC, Mao Y, Ranchalis J, Mallory BJ, Min AT, Hamm MO, Swanson E, Finkbeiner C, Li T, Whittington D, Noble WS, Stergachis AB, Vollger MR.<\/span> <span class=\"docsum-journal-citation full-journal-citation\"><em>bioRxiv.<\/em> 2023 Jul 6:2023.04.20.537673. doi: 10.1101\/2023.04.20.537673. Preprint.<\/span><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36837861\/\">The Effects of Pregnancy on Amino Acid Levels and Nitrogen Disposition.<\/a> Enthoven LF, Shi Y, Fay EE, Moreni S, Mao J, Honeyman EM, Smith CK, Whittington D, Brockerhoff SE, Isoherranen N, Totah RA, Hebert MF. <em>Metabolites.<\/em> 2023 Feb 7;13(2):242. doi: 10.3390\/metabo13020242.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Located in I-court (I &#8211; 093) of the Health Sciences Building on the University of Washington campus (click here for directions), the Mass Spectrometry Center is an open-access self-sustaining instrument laboratory providing a wide&#8230;<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":19275,"menu_order":98,"comment_status":"closed","ping_status":"closed","template":"templates\/template-medchem.php","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-270","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Mass Spectrometry Center<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/staff.washington.edu\/twentzel\/wordpress\/department-of-medicinal-chemistry\/research\/mass-spectrometry-center\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" 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