
Andy Hoofnagle MD PhD

Academic Title Assistant Professor Hospital positions Director, Clinical Mass Spectrometry Assistant Director, Clinical Chemistry Assistant Director, Clinical Immunology Associate Director, Clinical Nutrition Research Unit, Analytical Core

How to reach me
Mailing address: Andrew N. Hoofnagle, MD, PhD Department of Lab Medicine Campus Box 357110 University of Washington Seattle, WA 98195-7110	Courier address: Department of Lab Medicine 1959 NE Pacific St, Room NW120 University of Washington Seattle, WA 98195-7110
Main office: (206) 598-6131 Voicemail: (206) 598-4005 Fax: (206) 598-6189 Email: ahoof@u.washington.edu

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Research Interests
There are three main directions of research in our laboratory. The first investigates the interaction of inflammation and lipid metabolism using proteomics, mouse models, and cell-based methods. In addition, we are developing novel high-throughput immunoassays and quantitative mass spectrometric assays for biomarker validation in large studies. The second is the developmnent of clinical laboratory tests using liquid-chromatography/tandem mass spectrometry. Initially we are attempting to clinically translate peptide immunoaffinity methodologies to the detection in serum of the tumor marker thyroglobulin, the best marker we have for differentiated thyroid carcinoma. Lastly, we use informatics and data mining to develop useful benchmarks from large datasets of common laboratory tests to evaluate medical interventions throughout the hospital to optimize patient care and to ensure patient safety.

Available instrumentation and expertise
The Department of Laboratory Medicine can provide high-throughput analysis of human or animal specimens using LC-MS/MS. Currently, assays available include nicotine and its metabolites, 25-hydroxy vitamins D2 and D3 , plasma metanephrines, 8-isoprostanes, and the immunosuppresants cyclosporin, tacrolimus, and sirolimus. We are interested in working with other researchers in generating new assays with potential clinical utility. The unique workflow available through Research Testing Services and the Clinical Nutrition Research Unit make it easy to utilize the following instrumentation: - Three Waters Quattro Micro mass analyzers equipped with 2795 autosampler-HPLC units - A Waters Quattro Micro with an Acquity ultra-high pressure liquid chromotography front end - An Applied Biosystems API 4000 Q-TRAP with either normal flow (Shimadzu) or nanoflow (Eksigent) capability.

Other mass spectrometry at UWMC:
Jay Heinecke's Lab
Mike MacCoss's Lab
Medicinal Chemistry Mass Spectrometry Center
Proteomics at the Fred Hutchinson Cancer Research Center
Metabolomics User's Group

Selected publications

Hoofnagle AN, Heinecke JW. (2009) Lipoproteomics: using mass spectrometry-based proteomics to explore the assembly, structure, and function of lipoproteins. J Lipid Res. 50:1967-75. Hoofnagle AN, Wener MH. (2009) The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry. J Immunol Methods. 347:3-11. Amukele TK, Hoofnagle AN, Astion ML. (2009) Use of a resident on-call database to characterize failures in communicating critical laboratory results. Clin Chem. 55:1590-1. Simons SA, Molinelli AR, Sobhani K, Rainey PM, Hoofnagle AN. (2009) Two cases with unusual vancomycin measurements. Clin Chem. 55:578-80. Hoofnagle AN, Becker JO, Wener MH, and Heinecke JW. (2008) Quantification of thyroglobulin, a low-abundance serum protein, by immunoaffinity peptide enrichment and tandem mass spectrometry. Clin Chem. 54:1796-804. Marney LC, Laha TJ, Baird GS, Rainey PM, and Hoofnagle AN. (2008) Isopropanol protein precipitation for the analysis of plasma free metanephrines by liquid chromatography-tandem mass spectrometry. Clin Chem. 54:1729-32. Hoofnagle AN, Peterson GN, Kelly JL, Sayre CA, Chou D, and Hirsch IB. (2008) Use of serum and plasma glucose measurements as a benchmark for improved hospital-wide glycemic control. Endocr Pract. 14:556-63. Vaisar T, Pennathur S, Green PS, Gharib SA, Hoofnagle AN, Cheung MC, Byun J, Vuletic S, Kassim S, Singh P, Chea H, Knopp RH, Brunzell J, Geary R, Chait A, Zhao1 X, Elkon K, Marcovina S, Ridker P, Oram JF, and Heinecke JW. (2007) Shotgun Proteomics Implicates Protease Inhibition and Complement Activation in the Anti-inflammatory Properties of HDL. J Clin Invest. 117:746-56. Hoofnagle AN, Chou D, and Astion ML. (2007) Online Database for Documenting Clinical Pathology Resident Education. Clin Chem. 53:134-7. Hoofnagle AN, Laha TJ, Rainey PM, and Sadrzadeh SMH. (2006) Specific detection of anabasine, nicotine and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry. Am J Clin Path. 126:880-7. Pierce KM, Hoggard JC, Hope JL, Rainey PM, Hoofnagle AN, Jack RM, Wright BW, and Synovec RE. (2006) Fisher Ratio Method Applied to Third-Order Separation Data To Identify Significant Chemical Components of Metabolite Extracts. Anal Chem. 78:5068-75. Hoofnagle AN and Wener MH. (2006) Serum thyroglobulin: A model of immunoassay imperfection. Clin Lab Int. 12:12-4. Hoofnagle AN, Stoner JW, Lee T, Eaton SS, and Ahn, NG. (2004) Phosphorylation-dependent changes in structure and dynamics in ERK2 detected by SDSL and EPR. Biophys J. 86:395-403. Hoofnagle AN, Resing KA, and Ahn NG. (2003) Protein Analysis by Hydrogen Exchange Mass Spectrometry. Annu Rev Biophys Biomol Struct. 32:1-25. Emrick MA, Hoofnagle AN, Miller AS, Ten Eyck LF, and Ahn NG. (2001) Constitutive activation of extracellular signal-regulated kinase 2 by synergistic point mutations. J Biol Chem. 276:46469-79. Hoofnagle AN, Resing KA, Goldsmith EJ, and Ahn NG. (2001) Changes in protein conformational mobility upon activation of extracellular regulated protein kinase-2 as detected by hydrogen exchange. Proc Natl Acad Sci U S A. 98:956-61.
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